Common Reagents

Peptide Coupling Reagents

Peptide coupling reagents activate the acid group of the protected amino acids for forming a new peptide amide bond. These reagents are also often used to attach the first amino acid of a peptide sequence to the peptide synthesis resin. The most peptide coupling reagents belong to one of these categories:

  • BOP
  • BOP-Cl
  • PyBOP
  • PyAOP
  • PyBrOP
  • HBTU
  • HATU
  • HCTU
  • TATU
  • TBTU
  • TCFH
  • TNTU
  • TOTU
  • TPTU
  • TSTU
  • HOAt
  • HOBt
  • 6-Cl-HOBt
  • HOOBt
  • HOSu

Protecting/Labelling Reagents

Protecting groups are temporarily applied to keep reactive functional groups of the amino acids and peptides from interfering in the peptide synthesis reactions and producing unwanted side products. At the appropriate point in the synthesis, the protecting groups are removed to expose the functional group.

  • 7-Amino-4-methylcoumarin (AMC)
  • Biotin
  • BOC-ON
  • DMT-Cl
  • 9-Fluorenylmethanol (Fm-OH)
  • Fmoc-OSu
  • Fmoc-Cl
  • Fmoc-NH2
  • TBDMS-Cl
  • Trityl Chloride
  • Z-OSu
  • Z(2-Br)-OSu
  • Z(2-Cl)-OSu